CN EN

Media Formulation
Development

Different cell lines, including CHO, HEK 293, insect cells, and others, have unique nutritional requirements. Standard commercial cell culture media often fail to meet these specific demands, which can impact protein yields and quality. Additionally, relying on a single supplier can lead to challenges in controlling costs, delivery times, and long-term supply stability.

JSBio is committed to developing "customized cell culture media formulations" tailored specifically for your unique cell lines. Our customized solutions can help you achieve:

Our advantages

  • 1

    Enhanced Platform Performance

    Increase protein yield and quality (Increased by at least twofold)

  • 1

    Confidentiality Assurance

    Strict confidentiality agreements to protect your proprietary formulations and data.

  • 1

    Optimal Cost Efficiency

    Economies of scale through bulk raw material purchases and large-scale production.

  • 1

    Stable Product Supply

    Delivery schedules that meet your needs.

  • 1

    Minimized Risk

    Lifetime guarantee for the intellectual property rights of your exclusive formulation.

Development Process

Information interaction
  • Cell line
  • >Expression system
  • Current media
  • Current culture process
  • Project milestone plan
  • Expected goal
  • Experimental scheme
Screening and evaluation of platform media
  • Identify potential platform media
  • Identify key components that affect cell growth, protein expression and protein quality
Further optimization
  • Further optimize the key components that affect cell growth, protein expression and protein quality, including single factor study, multi-factor DOE experiment.
Results evaluation, technology transfer and process verification
  • Development and scale-up of shake flask,TPP tube and 2L bioreactor processes

Service Pattern & Deliverables

Service pattern Formulation development fully entrusted to JSBio Formulation development by customer itself
Deliverables Technical report on culture medium development and optimization
Technology transfer support
Commissioned production 		Experimental scheme
Platform media
Feeding reagent
Data analysis
Technical support
Commissioned production

Related Case

Antibody Case，Fed-batch process
Compared with a commercial medium, the application of the cell culture process developed by JSBio, paired with its proprietary medium formulation achieved superior cell growth and better maintenance of viable cell viability over a 14-day culture period. It ultimately boosted the protein titer to 10–11 g/L, 2–3 times that of the control group, while ensuring product quality fully complied with predefined specifications.
Antibody Case，Perfusion process
A perfusion medium and corresponding perfusion culture process were developed for the client. Compared to the control, our perfusion medium enabled higher and more stable harvest titers, approximately 2.5 g/L/day, while simultaneously reducing the accumulation of product within the bioreactor.
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