CN EN

Druggability evaluation platform

In Silico predictive characteristics evaluation
  • Predicted pl of Fv
  • Predicted pl of VH
  • Predicted pl of VL
  • Predicted pl of intact protein
  • Total CDR length
  • CDR variable sites
  • Neo CDR PSH score
  • Near CDR PPC score
  • Near CDR PNC score
  • StICSP score
  • Excess sulfhydryl
  • Excess glycosylation sites
  • GRAVY (hydrophilic)
  • CamSol_vl (solubility)
  • CamSolvh (solubility)
Molecular physicochemical and structural properties
  • Molecular size purity (SEC-HPLC)
  • Molecular Size Purity (iCIEF)
  • Molecular Size Purity (CE-SDS)
  • Isoelectric point (cIEF)
  • Purity of charge (cIEF)
  • Solubility (PEG method)
  • Solubility (ultrafiltration)
  • Viscosity
  • Colloidal stability (kD)
  • Colloidal stability (B22)
  • Thermal stability (Tm)
  • Thermal stability (Tonset)
  • Particle size (DLS)
  • Molecular weight
    Mass spectrum (RP-MS)
  • Mass spectrum peptide map
    (RP-HPLC/MS)
  • Self-interaction (Ac-SINS)
  • Hydrophobicity (HIC-HPLC)
Mimicking in vivo properties
  • Nonspecific binding
    (PSP)
  • Nonspecific binding
    (BVP)
  • Nonspecific binding
    (CHO cell binding)
  • Nonspecific binding
    (293T cell binding)
  • PK related properties
    (heparin column binding)
  • PK related properties
    (fetuin affinity with all method)
  • PK related properties
    (pH 6.0/7.4 FcRn binding)
  • PK characteristics analysis
    (ELISA or MS)
  • Immunogenicity
    (PBMC)
  • Plasma stability analysis
    (SEC/GXHPLC)
Preliminary stability properties
  • High temperature stability (45 °C, T0,1 wk, 2 wk)
  • Light stability (1.2×10⁶ lux·h, T0/T5/T10)
  • Strong oxidizer stability (0.02%H2O2, T0/T5/T10)
  • Low pH stability (pH3.0, 1, 2h, RT)
  • High pH stability (pH8.0, 10.0, 0.5h, RT)
  • Freeze-thaw stability (-80 °C/RT, 3 cycles)
  • Shake stability (200 rpm, T0, T5, T10)
Prediction In Silico
HIC-HPLC
Heparin-HPLC
BVP ELISA
B22
Forced degradation studies (PTMs)
DAR
Potency